Prevention of Stroke in Nonvalvular Atrial Fibrillation.

Cerebro-vascular Disease (CVD) is the third most common cause of death in developed world after cancer and IHD. Atrial fibrillation (AF) is responsible in 45% of cases cardioembolism leading to CVD. Atrial fibrillation is considered to be one of the growing cardiovascular epidemics in the 21st century. Warfarin is a proven drug for prevention of stroke in patients with atrial fibrillation. Newer anticoagulants are being tried, fibrillation. Newer anticoagulants are being tried, but not yet well established by clinical trials. Separation of left atrial appendage from circulation by surgery or device implantation is a promising one in this field.

Hepatitis C prevalence and risk behavior of injecting drug users in Sydney: a continuing concern.

This study examines patterns of injecting drug use and hepatitis C (HCV) risk behavior among injecting drug users. A survey of injecting drug users attending needle and syringe programs (NSPs) in the Sydney metropolitan area and members of NSW Users and AIDS Association (NUAA) yielded 336 responses. Demographic, behavioral and drug-use information was collected from injecting drug users aged from 14 to 64 years. The majority of respondents (66%) were HCV positive, 28% had tested negative and 5% did not know their status. Prevalence was higher among men than among women (54% vs 44%). Two thirds of respondents (72%) reported frequent heroin injection. Multivariate analysis identified the following significant risk factors for hepatitis C: being more than 30 years of age, an injecting history of five years or more, and having shared drug injecting equipment with a HCV positive user. The most significant factor associated with needle sharing was having unprotected sex with sexual partners and having a positive hepatitis C test result. Early identification of these factors should be a component of HCV prevention programs. Our data indicate that the promotion of safer injecting continues to be an important public health issue with regard to reducing HCV infections.

Baculovirus vector-mediated expression of heterologous genes in insect cells.

The baculovirus expression system employing Autagrapha californica nuclear polyhidrosis virus and Spodoptera frugiperda insect cells in culture has proved very popular for high level expression of heterologous genes: In this system, transcription of the foreign gene is usually driven by the hyperactive and temporally regulated polyhedrin gene promoter. Replacement of the polyhedrin gene, which encodes a 29-kDa occlusion protein (non-essential for viral replication), with a gene of interest leads to an occlusion negative phenotype which serves as a visual marker to select for recombinant viruses. Simultaneous expression of multiple genes can also be achieved. The heterologous proteins synthesized in this system are antigenically, immunologically and functionally identical in most respects to their native counterparts. This mini-review will aim at summarizing the potentials and utility of the baculovirus expression vector system and will address some important questions relating to the biology of this system.